Building a bridge to a new spindle pole body
نویسندگان
چکیده
منابع مشابه
Building a spindle pole
astillo et al. (page 453) have identified what may be a key event in building the spindle pole body (SPB) of budding yeast. They found that the kinase Mps1p phosphorylates and thus helps organize Spc42p, an important component of the SPB central plaque. Mps1p has been a difficult protein to study, as it does double duty as a regulator of SPB structure, and as part of the spindle checkpoint sign...
متن کاملDirect interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body
The Saccharomyces cerevisiae genes KAR1 and CDC31 are required for the initial stages of spindle pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KAR1 (Vallen, E. A., W. Ho. M. Winey, and M. D. Rose. 1...
متن کاملDirect Interaction between Yeast Spindle Pole Body Components: Karlp Is Required for Cdc31p Localization to the Spindle Pole Body
The Saccharomyces cerevisiae genes KAR/ and CDC31 are required for the initial stages of spindie pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calciumbinding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KARl (VaUen, E. A., W. Ho, M. Winey, and M. D. Rose. 199...
متن کاملThe Saccharomyces cerevisiae spindle pole body is a dynamic structure.
During spindle pole body (SPB) duplication, the new SPB is assembled at a distinct site adjacent to the old SPB. Using quantitative fluorescence methods, we studied the assembly and dynamics of the core structural SPB component Spc110p. The SPB core exhibits both exchange and growth in a cell cycle-dependent manner. During G1/S phase, the old SPB exchanges approximately 50% of old Spc110p for n...
متن کاملComponents of the yeast spindle and spindle pole body
Yeast spindle pole bodies (SPBs) with attached nuclear microtubles were enriched approximately 600-fold from yeast cell extracts. 14 mAbs prepared against this enriched SPB fraction define at least three components of the SPB and spindle. Immunofluorescent staining of yeast cells showed that throughout the cell cycle two of the components (110 and 90 kD) were localized exclusively to the SPB re...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2015
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.2096iti1